May 29, 2020 , The RNA-binding protein HuR posttranscriptionally regulates IL-2 homeostasis and CD4+ Th2 differentiation. Immunohorizons 1, 109–123 (
2020-01-10 · Human antigen R (HuR) is an RNA binding protein that promotes mRNA stability. Here the authors show that HuR represses adipogenesis in white and brown adipose tissue by stabilizing Insig1 and
HuR is an RNA-binding protein that resides predominantly in the nucleus but shuttles to the cytosol carrying mRNAs containing a uridylate-rich region in the 3'untranslated region. RNA-binding protein HuR binds to and stabilizes OIP5-AS1. Among the vast collection of lncRNAs annotated for human sequences (9837 lncRNAs, Ensembl v72) (35, 36), OIP5-AS1 was identified as the human homolog of cyrano, a lncRNA that plays a role in zebrafish development , and showed significant conservation in gene structure . Poly(C) Binding Proteins (CP1 and CP2). These RNA-binding proteins regulate mRNA stability (HuR) and mRNA turnover and translation (CP1 and CP2). The negative control RNA [poly(A) 25 RNA] does not contain HuR or poly(C) BP binding sites. Incubation of A431 lysate with labeled AR UTR RNA enriches HuR RBP; incubation with only poly(A) 25 RNA or HuR is a member of the embryonic lethal abnormal vision (ELAV) family of RNA-binding proteins .
HuR can stabilize target mRNAs and/or promote translation by interacting with their 39 untranslated region adenylate and uridylate–rich elements. Human antigen R (HuR) is an RNA-binding protein (RBP) belonging to the human embryonic lethal abnormal visual (ELAV) protein family that was originally identified as a specific antigen in patients with paraneoplastic neurological symptoms [ 1, 2 The RNA-binding protein (RBP) Hu-antigen R (HuR) is a central player in posttranscriptional regulation of cancer-related gene expression, and contributes to tumorigenesis, tumor growth, metastasis, and drug resistance. While many RNA‐binding proteins have been shown to bind to AREs in vitro, neither the functional consequences nor the physiological significance of their interactions are known. Here we demonstrate a role for the embryonic lethal abnormal visual (ELAV) RNA‐binding protein HuR in mRNA turnover in vivo. Background: The RNA-binding protein HuR is involved in a range of cellular processes and several diseases.Results: We reveal the characteristics of HuR binding using genomic methods and explore its network of targets.Conclusion: Our results reveal the complexity of RBP binding, corroborate the concept of post-transcriptional networks and suggest an interplay between miRNAs and RBPs Human antigen R (HuR) is an RNA binding protein that binds to the AU-rich element (ARE) of specific mRNA and is involved in the export and stabilization of ARE-mRNA. Our recent report unveiled that the E4orf6 gene deleted oncolytic adenovirus (dl355) replicated for certain types of cancers where ARE-mRNA is stabilized. The human embryonic lethal abnormal vision-like protein, HuR, is a member of the Hu family of RNA-binding proteins.
ISBN 9781597452281. Schumacher, Jill; Keesook Lee; Susanne Edelhoff; Robert Braun (May 1995). "Spnr, a Murine RNA-binding Protein That Is Localized to
RNA-binding proteins HuR and PTB promote the translation of hypoxia-inducible factor 1alpha. Mol Cell Biol 28, 93-107. Crossref, Medline, Google Scholar; Gallardo T, Shirley L, John GB, Castrillon DH (2007). Generation of a germ cell-specific mouse transgenic Cre line, Vasa-Cre.
Närmare bestämt beskrev RNA rullgardinsprotokoll nedan använder en RNA-fragment från AR 3'UTR som bete för att hämta HuR protein, en allmänt uttryckt
Even though the transcriptional activation of ABCA1 is well established, the posttranscriptional regulation of ABCA1 expression is poorly understood. Here, we investigate the potential contribution of the RNA binding protein (RBP) human antigen R (HuR) on the posttranscriptional regulation of ABCA1 expression HuR/ELAVL1 is an RNA-binding protein involved in differentiation and stress response that acts primarily by stabilizing messenger RNA (mRNA) targets. HuR comprises three RNA recognition motifs (RRMs) where the structure and RNA binding of RRM3 and of full-length HuR remain poorly understood.
Finns kvar introner mellan cap och exoner. 5' untranslated region.
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Over the past decade, this ubiquitously expressed protein has been extensively investigated in cancer research because it is involved in the regulation of mRNA stability and translation in many cell types. RNA-binding proteins (RBPs) play an essential role in governing the fate of mRNA transcripts from biogenesis, stabilization, translation to RNA decay 5, 6. Several RBPs have been reported as The RNA-binding protein HuR binds to elements rich in adenylate and uridylate (AU-rich elements) in target mRNAs and stabilizes them against degradation. The complete spectrum of genes whose expression is regulated by HuR and are the basis for the broad range of cellular functions of the protein is incompletely understood.
2020-01-10 · Human antigen R (HuR) is an RNA binding protein that promotes mRNA stability. Here the authors show that HuR represses adipogenesis in white and brown adipose tissue by stabilizing Insig1 and
ELAV-like protein 1 or HuR is a protein that in humans is encoded by the ELAVL1 gene.
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RNA-protein-interaktioner i bakteriella patogener on RNA-binding proteins will uncover virulence-specific regulatory RNAs and av nya regulatoriska mekanismer och öka den generella förståelsen för hur bakterier styr genuttryck. Eftersom
Post-transcriptional regulation of mRNA by the RNA-binding protein HuR (encoded by Elavl1) is required in B cells for the germinal center reaction and for the production of class-switched Human antigen R (HuR) is an RNA-binding protein that regulates the stability, translation, and nucleus-to-cytoplasm shuttling of its target mRNAs. While HuR is normally localized with ….
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RNA‐binding protein HuR suppresses senescence through Atg7 mediated autophagy activation in diabetic intervertebral disc degeneration 1 INTRODUCTION. Low back pain is a common symptom of various medical conditions, and up to 80% of the population may 2 MATERIAL AND METHODS. All treatments on
The protein encoded by this gene is a member of the ELAVL protein family. This encoded protein contains 3 RNA-binding domains and binds cis-acting AU-rich elements. One of its best-known functions is to stabilize mRNAs in order to regulate gene expression. 2018-04-20 · METHODS AND RESULTS: Herein, we show that, as evidenced by ribonucleoprotein immunoprecipitation assay, RNA binding protein Hu antigen R/ELAV like RNA binding protein 1 (HuR/ELAVL1) and myocyte enhancer factor-2C (MEF2C) transcription factor mRNA are associated. HuR positively regulated transcription factor MEF2C mRNA expression by protecting its mRNA from degradation.